Over 250k Biomarkers Tested

Cortisol 4pm Collection Time Blood Test

$55.00 AUD

Afternoon energy crashes, difficulty winding down in evening, or assessing your daily cortisol decline pattern?

Afternoon cortisol measurement capturing mid-decline in daily rhythm. Useful for assessing whether cortisol is dropping appropriately from morning peak.
Collection Location
Specimen Type

You will be emailed a referral to take to your local collection centre. If you ever have any questions, we're here to help.

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Getting your health measured shouldn't be hard! We're here to help you every step of the way.

1
Order a test

Order a test

Order the private test that suits you and your goals. After ordering, you will receive your referral by email.

2
Collect the sample

Collect the sample

Attend one of our 4000+ partner collection centres throughout Australia. Search locations.

3
Receive your results

Receive your results

View all your lab results in your secure health dashboard. Easy!

Overview

Cortisol 4pm testing captures your stress hormone level during its normal afternoon decline. Healthy adrenal function shows high morning cortisol gradually dropping through the day, reaching this mid-afternoon checkpoint significantly lower than morning peak.

This test helps assess whether your daily rhythm is functioning—afternoon crashes may reflect abnormally low cortisol, while evening anxiety or sleep difficulty may indicate cortisol that's not declining appropriately, available across Australia.

Most informative when compared to morning cortisol values to see the full pattern.

Symptoms

Essential for people with afternoon energy crashes, evening anxiety, sleep onset difficulty, or comprehensive adrenal rhythm assessment.

Questions

  • 4pm captures cortisol during its normal afternoon decline. Comparing afternoon to morning levels reveals whether your rhythm is functioning properly.
  • Afternoon cortisol should be significantly lower than morning levels—typically 50-70% lower. The decline matters more than absolute values.
  • Persistently elevated afternoon cortisol suggests chronic stress, Cushing's syndrome, or rhythm disruption. You may feel wired or anxious in evenings.
  • Very low afternoon cortisol with low morning levels suggests adrenal insufficiency. Low afternoon with normal morning may be normal variation.
  • Yes, comparing morning and afternoon levels provides more information than either alone. Some providers order both simultaneously.
  • High evening cortisol can interfere with sleep onset. The natural decline should reach lowest levels at bedtime.

Dr. Vu Tran
Bloody Good’s Chief Medical Officer

Biomarker Tested

How to prepare

After the test

Your test results will be available in your private dashboard. If there are any urgent issues, we'll let you know so you can follow up with your health professional.

Understanding results

Testimonials

What Our Customers Say

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Adam O. ACT

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Frequently Asked Questions

Most collection centres accept walk-ins. Some may require a booking - check details when you click on your chosen location.

If your test needs fasting, we’ll include that in your instructions after you order. Fasting usually means no food for 8–12 hours, but water is fine.

Just your pathology referral form (we email it to you)

Of course. Just maybe don’t bring the friend who faints at the sight of blood.

They’re the highly trained professionals who take your blood sample - with a steady hand and a sharp needle. They love blood, but don’t worry… they’re not vampires. Just legends who make blood tests quick, clean, and (almost) painless.

Some of our tests include Urine, Stool, Saliva and more. Each test will have a clear description on what sample you will need to give and instructions on how.