Over 250k Biomarkers Tested

Cortisol 4pm Collection Time Blood Test

$55.00 AUD

Afternoon energy crashes, difficulty winding down in the evening, or wanting to check your daily cortisol decline pattern?

Measures cortisol at 4pm, capturing mid-decline in the daily adrenal rhythm. Assesses whether cortisol is dropping appropriately from the morning peak. Most informative when paired with an 8am test.

Collection Location
Specimen Type

You will be emailed a referral to take to your local collection centre. If you ever have any questions, we're here to help.

Private health tests

How It Works

Getting your health measured shouldn't be hard! We're here to help you every step of the way.

1
Order a test

Order a test

Order the private test that suits you and your goals. After ordering, you will receive your referral by email.

2
Collect the sample

Collect the sample

Attend one of our 4000+ partner collection centres throughout Australia. Search locations.

3
Receive your results

Receive your results

View all your lab results in your secure health dashboard. Easy!

Overview

Cortisol 4pm testing captures your stress hormone level during its normal afternoon decline. Healthy adrenal function shows high morning cortisol gradually dropping through the day, reaching this mid-afternoon checkpoint significantly lower than the morning peak.

This test helps assess whether your daily cortisol rhythm is functioning as expected. Afternoon energy crashes may reflect lower-than-expected cortisol, while evening restlessness or sleep difficulty may suggest cortisol is not declining appropriately.

Most informative when compared to an 8am morning cortisol result to map the full daily pattern.

Symptoms

Suited to people experiencing afternoon energy crashes, difficulty winding down in the evening, sleep onset issues, or those wanting to assess their daily cortisol pattern.

Questions

  • 4pm captures cortisol during its normal afternoon decline. Comparing afternoon levels to morning levels shows whether your daily rhythm is functioning as expected.
  • Afternoon cortisol should typically be 50 to 70 percent lower than morning levels. The pattern of decline matters more than the absolute value.
  • Persistently elevated afternoon cortisol may suggest chronic stress, Cushing's syndrome, or disruption to your daily rhythm. Your doctor can advise on further investigation.
  • Very low afternoon cortisol alongside low morning levels may suggest adrenal insufficiency. Low afternoon with normal morning levels may be a normal variation.
  • Yes. Comparing morning and afternoon levels provides more useful information than either test alone. Some doctors order both at the same time.
  • Elevated evening cortisol can interfere with sleep onset. The natural decline should reach its lowest point around bedtime.

Dr. Vu Tran
Bloody Good’s Chief Medical Officer

Biomarker Tested

How to prepare

Blood must be collected at 4pm (or as close as possible). No fasting required. Avoid intense exercise, caffeine, and alcohol on the day of collection. Let the pathology collector know about any medications you are taking, especially corticosteroids.

After the test

Discuss results with your doctor. If you have not already tested morning cortisol, they may recommend an 8am collection for comparison. Abnormal afternoon levels may prompt further investigation of adrenal function or referral to an endocrinologist.

Your test results will be available in your private dashboard. If there are any urgent issues, we'll let you know so you can follow up with your health professional.

Understanding results

Results show your 4pm cortisol level compared to afternoon reference ranges. Afternoon cortisol should typically be 50 to 70 percent lower than morning values. Your healthcare provider will interpret results alongside any morning cortisol readings and your symptoms.

Testimonials

What Our Customers Say

4.9
Average Rating
5,500+ Customers Tested
250,000+ Individual Biomarkers
A
Adam O. ACT

Simple health checks in my control

I didn't know that private blood tests were an option. Tried this platform as the packages suited what I wanted for a good price. Easy to follow instructions, and super quick results. Platform provides more information than my gp's ever have on the tests and what the results meant. Would like more clinics to be able to test at (specifically Capital Pathology) but there is a decent network. Will continue to use.

The Performance Check
W
Wayne S. NSW

Bloody Good

This is a great service, very helpful support, cheaper than going to the doctor for a blood test

Vitamin D (25-OH)
C
Christopher N. NSW

Quick access to blood tests

BLOODY GOOD is a very efficient route to access blood testing for a visitor outside the Australian health system. It is quick with the necessary referral and documentation, and quick with results. Thank you

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Rebecca Y NSW

Great Service!

Fantastic service to deal with, I love the extra explanations for all the tests.

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Olga B. New South Wales

Convenient

Getting a referral was easy. Laverty pathology accepted it. Bloody Good offers an affordable and convenient way to check essential blood markers.

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Marita S. Queensland

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Lukas C. NSW

Quick, easy, hassle free

Excellent service, quick, easy, hassle free, can recommend.

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ABDUL S. QLD

Amazingly quick and efficient

Very easy to use. Love that it stores your blood tests.

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Frequently Asked Questions

Most collection centres accept walk-ins. Some may require a booking - check details when you click on your chosen location.

If your test needs fasting, we’ll include that in your instructions after you order. Fasting usually means no food for 8–12 hours, but water is fine.

Just your pathology referral form (we email it to you)

Of course. Just maybe don’t bring the friend who faints at the sight of blood.

They’re the highly trained professionals who take your blood sample - with a steady hand and a sharp needle. They love blood, but don’t worry… they’re not vampires. Just legends who make blood tests quick, clean, and (almost) painless.

Some of our tests include Urine, Stool, Saliva and more. Each test will have a clear description on what sample you will need to give and instructions on how.