How to prepare
- Collection timing: The four samples are collected at specific intervals across a single day. Your kit instructions will specify the recommended windows (typically waking, mid-morning, afternoon, and evening). Follow the timing guidance closely for accurate results.
- Food and drink: Avoid eating, drinking anything other than water, or using a straw for at least 30 minutes before each collection.
- Exercise: Avoid vigorous exercise for at least one hour before each sample.
- Dental hygiene: Do not brush your teeth immediately before collecting. Blood or inflammation in the mouth can affect cortisol results.
- Alcohol: Avoid for at least 12 hours before the testing day.
- Medications and supplements: Do not stop any medications. Inform your practitioner of any corticosteroids, DHEA supplements, or hormonal medications you are taking.
After the test
Address stress-immune imbalances through comprehensive stress management, immune-supporting supplementation, dietary modifications, gut health optimization, and lifestyle changes. Work with healthcare providers experienced in functional medicine or integrative immunology.
Your test results will be available in your private dashboard. If there are any urgent issues, we'll let you know so you can follow up with your health professional.
Understanding results
Your results will show cortisol levels at each of the four time points alongside DHEA and cortisone, with values compared to reference ranges.
- Four-point cortisol curve: A healthy pattern shows a high morning reading that declines progressively through the day. Flat curves, inverted patterns, or elevated evening cortisol each carry different clinical implications.
- DHEA: Reflects adrenal reserve and resilience. Low DHEA alongside low cortisol may suggest more significant adrenal fatigue. Elevated DHEA has its own considerations.
- Cortisone: Cortisone is the inactive form of cortisol. The ratio of cortisol to cortisone can indicate how efficiently your body is converting and using cortisol at the cellular level.
Your practitioner will interpret all markers together rather than in isolation.