Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test
Active Vitamin D (1,25 Dihydroxy) Blood Test

Over 250k Biomarkers Tested

Active Vitamin D (1,25 Dihydroxy) Blood Test

$67.00 AUD

Got persistently high calcium results but a normal standard vitamin D test, and want to understand what's going on with your vitamin D metabolism?

Measures 1,25-dihydroxyvitamin D (calcitriol), the active hormonal form of vitamin D produced by the kidneys. Not a routine vitamin D screening test; used to investigate calcium metabolism disorders, chronic kidney disease, and conditions like sarcoidosis.

Collection Location
Specimen Type

You will be emailed a referral to take to your local collection centre. If you ever have any questions, we're here to help.

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How It Works

Getting your health measured shouldn't be hard! We're here to help you every step of the way.

1
Order a test

Order a test

Order the private test that suits you and your goals. After ordering, you will receive your referral by email.

2
Collect the sample

Collect the sample

Attend one of our 4000+ partner collection centres throughout Australia. Search locations.

3
Receive your results

Receive your results

View all your lab results in your secure health dashboard. Easy!

Overview

The Active Vitamin D test measures calcitriol, which is like vitamin D's "final boss" – the most powerful form your body creates after processing regular vitamin D through your kidneys. Think of it as your body's master key for unlocking calcium absorption and bone strength. While regular vitamin D tests show how much vitamin D you're storing, this test reveals whether your body can actually activate and use it effectively, which is crucial for building strong bones, supporting your immune system, and maintaining healthy calcium levels.

This test becomes especially valuable when something seems off with your calcium levels or bone health, but standard vitamin D tests look normal. It's particularly helpful for people with kidney problems (since kidneys do the heavy lifting in vitamin D activation), unexplained high calcium levels, or complex bone disorders. If your results show low active vitamin D, it might mean your kidneys aren't converting stored vitamin D properly, while unusually high levels could indicate certain inflammatory conditions that overproduce this hormone-like vitamin.

Symptoms

To measure 1,25-dihydroxyvitamin D (calcitriol) in the context of calcium metabolism disorders, kidney disease, or granulomatous conditions, rather than for routine nutritional vitamin D screening.

Questions

  • Calcitriol (1,25-dihydroxyvitamin D) is the hormonally active form of vitamin D, produced by the kidneys from 25-OH vitamin D (the form measured in the standard vitamin D test). The 25-OH form is the storage and transport form and reflects overall nutritional vitamin D status. Calcitriol is the form that actually acts on cells — primarily to regulate calcium absorption. It's tightly controlled by the body and doesn't directly reflect dietary intake or sun exposure.
  • This test is typically ordered when investigating abnormal calcium levels, chronic kidney disease (where the kidneys' ability to activate vitamin D may be impaired), hyperparathyroidism, or granulomatous conditions like sarcoidosis, tuberculosis, or certain lymphomas that can produce calcitriol autonomously and cause elevated calcium.
  • This test is not the right tool for that purpose. For routine assessment of vitamin D nutritional status and supplementation monitoring, the 25-OH vitamin D test is the appropriate choice. This active vitamin D test serves a different clinical purpose.
  • The kidneys are responsible for converting 25-OH vitamin D into active calcitriol. In chronic kidney disease, this conversion is impaired, which can lead to low calcitriol levels. This contributes to problems with calcium and phosphate balance, weakened bones (renal osteodystrophy), and elevated parathyroid hormone. Measuring calcitriol directly can help characterise the degree of this impairment.
  • Yes. Conditions like sarcoidosis can cause immune cells (macrophages) to produce calcitriol independently of the normal kidney-regulated pathway. This can elevate calcitriol and lead to high blood calcium (hypercalcaemia) even when the standard 25-OH vitamin D test appears within range. This is one of the key reasons this test is used in the investigation of unexplained hypercalcaemia.

Dr. Vu Tran
Bloody Good’s Chief Medical Officer

Biomarker Tested

How to prepare

No specific fasting required. You can eat and drink normally before this test. If you take vitamin D supplements or medications affecting calcium metabolism, note these when discussing results with your practitioner.

After the test

Results from this test are best reviewed with a GP or specialist (such as an endocrinologist or nephrologist) who can consider them alongside your other markers and clinical history. Abnormal calcitriol levels without a clear cause warrant further investigation rather than self-directed supplementation.

Your test results will be available in your private dashboard. If there are any urgent issues, we'll let you know so you can follow up with your health professional.

Understanding results

Results are reported as calcitriol concentration (pmol/L) alongside a reference range. Elevated calcitriol can occur in kidney disease, sarcoidosis, other granulomatous diseases, and certain lymphomas. Low calcitriol is associated with advanced kidney disease, as the kidneys lose the ability to activate 25-OH vitamin D to its active form. Because calcitriol is tightly regulated, it can be normal even when 25-OH vitamin D is low, and vice versa. Interpretation alongside calcium, phosphate, PTH, and 25-OH vitamin D provides the fullest picture.

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What Our Customers Say

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Frequently bought together

Frequently Asked Questions

Most collection centres accept walk-ins. Some may require a booking - check details when you click on your chosen location.

If your test needs fasting, we’ll include that in your instructions after you order. Fasting usually means no food for 8–12 hours, but water is fine.

Just your pathology referral form (we email it to you)

Of course. Just maybe don’t bring the friend who faints at the sight of blood.

They’re the highly trained professionals who take your blood sample - with a steady hand and a sharp needle. They love blood, but don’t worry… they’re not vampires. Just legends who make blood tests quick, clean, and (almost) painless.

Some of our tests include Urine, Stool, Saliva and more. Each test will have a clear description on what sample you will need to give and instructions on how.