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Progesterone Blood Test

$55.00 AUD

Trying to understand whether you're ovulating, or working through unexplained fertility concerns? A timed progesterone test gives your practitioner direct evidence of whether ovulation has occurred.

The Progesterone Blood Test measures serum progesterone, the hormone produced after ovulation that maintains the uterine lining and supports early pregnancy. Tested at mid-luteal phase (typically day 21 of a 28-day cycle), a result above the reference threshold confirms that ovulation has occurred.

Collection Location
Specimen Type

You will be emailed a referral to take to your local collection centre. If you ever have any questions, we're here to help.

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1
Order a test

Order a test

Order the private test that suits you and your goals. After ordering, you will receive your referral by email.

2
Collect the sample

Collect the sample

Attend one of our 4000+ partner collection centres throughout Australia. Search locations.

3
Receive your results

Receive your results

View all your lab results in your secure health dashboard. Easy!

Overview

Progesterone is a steroid hormone produced primarily by the corpus luteum in the ovary after ovulation. Its main role is to prepare and maintain the uterine lining for a potential pregnancy. If ovulation does not occur, progesterone remains low throughout the cycle. If ovulation does occur, progesterone rises sharply in the luteal phase (the second half of the cycle) and peaks around 7 days after ovulation.

This makes a timed progesterone blood test one of the most direct ways to confirm that ovulation has occurred. For a standard 28-day cycle, day 21 captures the expected mid-luteal peak. For cycles that are shorter or longer than 28 days, the timing adjusts accordingly. The key is to test approximately 7 days after suspected ovulation, which is when the result will be most informative.

A mid-luteal progesterone above the laboratory's reference threshold is generally accepted as evidence of ovulation. A low result on a correctly timed sample may indicate anovulation (no ovulation occurred in that cycle) or luteal phase insufficiency, where the corpus luteum does not produce adequate progesterone. Both of these findings are relevant to fertility investigations and may prompt further assessment.

Progesterone is also monitored in early pregnancy, where adequate levels are needed to support the developing pregnancy until the placenta takes over hormone production. In assisted reproduction, progesterone supplementation is often used to support the luteal phase, and testing may be part of that monitoring.

Outside of reproductive contexts, progesterone is produced in small amounts by the adrenal glands in both men and women, and serves as a precursor to other hormones. However, the primary clinical use of this test is in the assessment of ovulation and female reproductive function.

Symptoms

Suited to women investigating whether they are ovulating, those experiencing irregular cycles or luteal phase concerns, women with unexplained infertility, and those in early pregnancy where progesterone monitoring is appropriate. Also used in assisted reproduction to assess luteal phase support.

Questions

  • Day 21 corresponds to the mid-luteal phase of a standard 28-day cycle, approximately 7 days after ovulation. This is when progesterone is expected to peak if ovulation has occurred. Collecting the sample at this time gives the highest chance of detecting a meaningful progesterone rise. If your cycle is not 28 days, the timing adjusts accordingly — the key is to test 7 days after expected ovulation.
  • For irregular cycles, testing 7 days after ovulation is more reliable than counting from the start of your period. Ovulation predictor kits can help identify when ovulation occurs, and you would then schedule the blood test 7 days after a positive result. If your cycles are very unpredictable, discuss timing with your practitioner before booking.
  • A low mid-luteal progesterone on a correctly timed sample suggests that ovulation may not have occurred, or that the luteal phase is producing less progesterone than expected. This can be associated with anovulatory cycles, luteal phase deficiency, thyroid conditions, hyperprolactinaemia, or significant physical stress. Your practitioner will advise on appropriate next steps.
  • Yes. A mid-luteal progesterone above the laboratory's reference threshold for ovulation confirmation is considered evidence that ovulation has occurred. It is one of the most direct methods available. Combine it with basal body temperature tracking or ovulation predictor kits for a more complete picture.
  • No. Fasting is not required for progesterone testing. The key preparation is timing — the test needs to be done at the right point in your cycle to produce a meaningful result.
  • Progesterone is produced in small amounts in men, primarily by the adrenal glands and testes, and is a precursor to other hormones including testosterone. It is occasionally measured in men as part of an adrenal or hormone investigation, but this is uncommon. This test is primarily used in the context of female reproductive health.

Dr. Vu Tran
Bloody Good’s Chief Medical Officer

Biomarker Tested

How to prepare

No fasting is required. Eat and drink normally beforehand.

Timing is the most important factor for this test. For a 28-day cycle, collection on day 21 captures the expected mid-luteal peak. If your cycle is shorter or longer than 28 days, collection should be approximately 7 days after you expect ovulation to have occurred. If you use ovulation predictor kits, this test is ideally done 7 days after a positive result.

Testing at the wrong time in your cycle will produce a misleading result. If you are unsure when to test, ask your practitioner before booking. A healthcare professional will take a blood sample from a vein in your arm.

After the test

Share your results with your GP, gynaecologist, or fertility specialist. If mid-luteal progesterone is low on a correctly timed sample, your practitioner will discuss whether further investigation is appropriate — this may include repeat testing, additional hormone markers, or a referral to a reproductive specialist.

Do not adjust medications or supplement with progesterone based on this result alone without consulting your practitioner.

Your test results will be available in your private dashboard. If there are any urgent issues, we'll let you know so you can follow up with your health professional.

Understanding results

Results are reported in nanomoles per litre (nmol/L). Reference ranges are cycle-phase specific. A mid-luteal progesterone above approximately 25–30 nmol/L is generally taken as evidence of ovulation, though reference ranges vary between laboratories.

A result below this threshold on a correctly timed sample may indicate that ovulation did not occur, or that the luteal phase is inadequate. Low progesterone in early pregnancy may also be of concern to your practitioner and may prompt further monitoring.

Because progesterone levels fluctuate over the course of the day, a borderline result may sometimes be repeated. Your practitioner will interpret the number in the context of your cycle length, timing, and clinical picture.

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Frequently Asked Questions

Most collection centres accept walk-ins. Some may require a booking - check details when you click on your chosen location.

If your test needs fasting, we’ll include that in your instructions after you order. Fasting usually means no food for 8–12 hours, but water is fine.

Just your pathology referral form (we email it to you)

Of course. Just maybe don’t bring the friend who faints at the sight of blood.

They’re the highly trained professionals who take your blood sample - with a steady hand and a sharp needle. They love blood, but don’t worry… they’re not vampires. Just legends who make blood tests quick, clean, and (almost) painless.

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