Immune & Allergy - Bloody Good
Vitamins & Minerals

Vitamin D (25-OH)

Boost your health from the inside out with our comprehensive Vitamin D Blood Test. Often called the 'sunshine vitamin', vitamin D is crucial for bone strength, immune function, mood regulation, and overall wellbeing - yet deficiency is surprisingly common, especially in Australia's climate extremes.

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Vitamin D (25-OH)

Wondering if low energy, frequent infections, mood changes, or bone concerns could be linked to your vitamin D levels, especially during winter months or with limited sun exposure?

Overview

Vitamin D is much more than just a vitamin - it's actually a powerful hormone that acts like your body's master conductor, orchestrating everything from bone strength to immune defense. While your skin naturally produces vitamin D when exposed to sunlight, modern life often keeps us indoors, and even in sunny Australia, deficiency is surprisingly common due to sunscreen use, office jobs, and seasonal changes.

This essential hormone works behind the scenes in remarkable ways: it unlocks your body's ability to absorb calcium for strong bones, fine-tunes your immune system to fight infections while keeping it from attacking healthy tissues, supports muscle strength and function, and even influences your mood and brain health. When your vitamin D levels are too low, you might experience fatigue, frequent infections, bone pain, or mood changes - your body's way of signaling that this crucial conductor is missing from the orchestra. Testing your vitamin D levels gives you valuable insight into whether your body has enough of this master regulator to keep all your systems performing in harmony.

Reviewed by Dr. Vu Tran
Bloody Good’s Chief Medical Officer

Symptoms

You may benefit from this test if you experience:
Bone pain or deep aching Muscle weakness in arms or legs Muscle pain after exercise that lasts days Fractures with minimal trauma Feeling tired all the time Feeling sad or down most of the time Frequent infections (more than 4 colds per year) Osteoporosis screening Feeling weak or feeble Feeling unsteady on feet Feeling hopeless about the future Joint pain that's worse in cold weather Knee pain when walking or climbing stairs Hip pain or stiffness Chronic lower back pain Shoulder pain or limited range of motion Difficulty climbing stairs due to leg weakness Tender points or trigger points Difficulty lifting objects you used to lift easily Depression or mood changes Bone pain or frequent fractures Supplement effectiveness monitoring Increased fall risk

Purpose

Essential for everyone, particularly those with limited sun exposure, office workers, people with darker skin, elderly individuals, and those living in southern Australia. Important for people experiencing frequent infections, mood changes, bone pain, muscle weakness, or fatigue. Valuable for monitoring supplementation effectiveness and optimizing immune function.

How to prepare

No fasting required. No special preparation needed. Can be taken at any time of day. If taking vitamin D supplements, maintain your usual routine unless specifically advised otherwise by your healthcare provider. Biotin supplements should be avoided for 3 days before testing.

Understanding results

Optimal levels: 75-150 nmol/L (30-60 ng/mL). Deficient: <50 nmol/L (<20 ng/mL); Insufficient: 50-74 nmol/L (20-29 ng/mL); Sufficient: ≥75 nmol/L (≥30 ng/mL). Toxicity rare but possible above 375 nmol/L (150 ng/mL). Many health experts recommend maintaining levels above 100 nmol/L (40 ng/mL) for optimal health benefits.

After the test

If levels are low, discuss appropriate supplementation with your healthcare provider. Typical recommendations include vitamin D3 (cholecalciferol) supplements, safe sun exposure, and dietary sources. Dosing depends on current levels, body weight, and individual factors. Retest in 3-6 months to monitor response to supplementation. Severe deficiency may require prescription-strength doses initially.

Frequently Asked Questions

  • In summer, 5-15 minutes of midday sun exposure to arms and face several times per week is usually sufficient for fair-skinned individuals. Darker skin requires longer exposure. Winter sun in southern Australia is often insufficient.
  • It's difficult to get adequate vitamin D from food alone. Fatty fish, eggs, and fortified foods help, but supplements are often necessary, especially in winter or with limited sun exposure.
  • Vitamin D3 (cholecalciferol) is more effective at raising and maintaining blood levels than vitamin D2 (ergocalciferol). Most supplements and recommendations focus on D3.
  • With appropriate supplementation, levels typically improve within 6-12 weeks, though it may take 3-6 months to reach optimal levels depending on starting point and dose.
  • Yes, excessive supplementation can lead to toxicity, causing nausea, kidney problems, and calcium buildup. However, toxicity is rare and usually requires very high doses over extended periods.
  • For most people, annual testing is sufficient. More frequent testing may be needed when starting supplementation, during treatment for deficiency, or if you have conditions affecting absorption.
Vitamin D (25-OH)

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1

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2

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3
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