Immune & Allergy - Bloody Good
Vitamins & Minerals

Active Vitamin D (1,25 Dihydroxy) Test

The Active Vitamin D Test, also called the Vitamin D (1,25 Dihydroxy) or calcitriol test, measures the active form of vitamin D that your body uses. This test may help check calcium balance, bone strength, muscle health, and immune function, especially when standard vitamin D tests don’t give the full picture.

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Active Vitamin D (1,25 Dihydroxy) Test

Concerned about bone health, frequent infections, or wondering if your body is properly activating vitamin D for optimal immune function and calcium absorption?

Overview

The Active Vitamin D test measures calcitriol, which is like vitamin D's "final boss" – the most powerful form your body creates after processing regular vitamin D through your kidneys. Think of it as your body's master key for unlocking calcium absorption and bone strength. While regular vitamin D tests show how much vitamin D you're storing, this test reveals whether your body can actually activate and use it effectively, which is crucial for building strong bones, supporting your immune system, and maintaining healthy calcium levels.

This test becomes especially valuable when something seems off with your calcium levels or bone health, but standard vitamin D tests look normal. It's particularly helpful for people with kidney problems (since kidneys do the heavy lifting in vitamin D activation), unexplained high calcium levels, or complex bone disorders. If your results show low active vitamin D, it might mean your kidneys aren't converting stored vitamin D properly, while unusually high levels could indicate certain inflammatory conditions that overproduce this hormone-like vitamin.

Reviewed by Dr. Vu Tran
Bloody Good’s Chief Medical Officer

Symptoms

You may benefit from this test if you experience:
Bone pain or deep aching Muscle weakness in arms or legs Fractures with minimal trauma Feeling tired all the time Feeling sad or down most of the time Frequent infections (more than 4 colds per year)

Purpose

Essential for individuals with kidney disease, complex vitamin D disorders, calcium abnormalities, or anyone needing detailed vitamin D metabolism and function assessment.

How to prepare

No special preparation required. Inform healthcare provider of all vitamin D supplements, calcium medications, and treatments that might affect vitamin D metabolism and active vitamin D levels.

Understanding results

Results show active vitamin D levels compared to normal ranges. Abnormal levels may indicate vitamin D metabolism disorders, kidney problems, or calcium regulation issues requiring specialized evaluation.

After the test

Address abnormal active vitamin D through specialized endocrine or nephrology evaluation, appropriate vitamin D treatments, calcium management, and regular monitoring. Work with specialists as needed.

Frequently Asked Questions

  • Active vitamin D (calcitriol) is the most potent form of vitamin D that regulates calcium absorption, bone metabolism, immune function, and cell growth throughout the body.
  • Regular vitamin D tests measure storage form (25-hydroxyvitamin D), while active vitamin D measures the most potent, hormonally active form that directly affects calcium and bone metabolism.
  • High levels may indicate granulomatous diseases, certain cancers, vitamin D intoxication, or conditions causing excessive vitamin D activation, requiring specialized evaluation.
  • Low levels may indicate kidney disease, genetic disorders affecting vitamin D metabolism, or problems with vitamin D activation, often requiring specialized treatment.
  • It's typically ordered for kidney disease, complex calcium disorders, granulomatous diseases, unexplained hypercalcemia, or when standard vitamin D testing is insufficient.
  • Treatment may include active vitamin D medications (calcitriol), addressing underlying kidney or metabolic problems, and careful monitoring of calcium levels.
Active Vitamin D (1,25 Dihydroxy) Test

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What to expect

We want you to feel bloody good—inside and out. Regular testing isn’t just about checking your current health; it helps you spot abnormal values you might not know about.

1

Book your referral

Choose the testing that suits your goals. After ordering, you will receive your referral by email.

2

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3
Receive your results

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View your lab results and receive a personalised review from the secure Bloody Good platform.

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